Get Help Sign In

Frequently asked questions

Our Scientific Applications Support team has assembled a list of frequently asked questions to help you find answers quickly. Filter using one or more categories to focus on specific topics, or use the search bar to perform a text search.

Is it OK to resuspend and store oligos in DEPC treated water?

No, we do not recommend resuspending or storing oligos in DEPC treated water. DEPC treated water can be acidic which may cause depurination of the oligo resulting in degradation.

For optimal stability, oligos that are to be stored long term should be stored frozen, at–20°C. If the oligos are going to be resuspended, storage in a TE buffer (10 mM Tris pH 8.0, 0.1 mM EDTA; such as IDTE) is better than using water. The impact from the type of storage medium used is marginal at -20°C, but becomes more important at higher temperatures (see Figures 1–3 above).

It is ideal to store oligos in the dark. Exposure to UV light should be avoided, and ambient lab light should be minimized, particularly for some types of modified oligos. Also, avoid unnecessary freeze thaw cycles by preparing a freezer stock solution (100 µM) and several working aliquots.

Use the IDT Resuspension Calculator under the "SciTools®" tab of the IDT website for help determining volumes to use.

Custom DNA & RNA

*RUO—For research use only. Not for use in diagnostic procedures. Unless otherwise agreed to in writing, IDT does not intend for these products to be used in clinical applications and does not warrant their fitness or suitability for any clinical diagnostic use. Purchaser is solely responsible for all decisions regarding the use of these products and any associated regulatory or legal obligations.