Is it OK to resuspend and store oligos in DEPC treated water?
No, we do not recommend resuspending or storing oligos in DEPC treated water. DEPC treated water can be acidic which may cause depurination of the oligo resulting in degradation.
We recommend that oligos are stored long term at at ‒20°C. If the oligos are going to be resuspended, storage in a TE buffer such as IDTE (10 mM Tris pH 8.0, 0.1 mM EDTA) is better than using water. The impact from the type of storage medium used is marginal at ‒20°C but becomes more important at higher temperatures.
It is ideal to store oligos in the dark. Exposure to UV light should be avoided and ambient lab light should be minimized, particularly for some types of modified oligos. Also, avoid unnecessary freeze-thaw cycles by preparing a freezer stock solution (100 µM) and several working aliquots.
Use the IDT Resuspension Calculator under the SciTools™ tab of the IDT website for help determining volumes to use.