Frequently asked questions

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Is it possible for the Alt-R® Cas9 Electroporation Enhancer to integrate at the Cas9 cut site?

Our next generation sequencing data demonstrated a very low rate of integration of the enhancer into the target site (less than 1 in 10,000 reads, which is much lower than the insertion rate of random pieces of cellular DNA). Therefore, enhancer integration should not negatively impact experimental outcomes.