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Is the Alt-R® CRISPR-Cas9 ribonucleoprotein (RNP) complex stable? Do I need to make a fresh complex for each experiment?
The Alt-R® Cas9 RNP complex is stable when stored at –80°C or 4°C for 6 months (stability testing is ongoing), so you do not have to make the RNP fresh for each experiment.
We do not recommend storage of the RNP complex at –20°C.