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Frequently asked questions

Our Scientific Applications Support team has assembled a list of frequently asked questions to help you find answers quickly. Filter using one or more categories to focus on specific topics, or use the search bar to perform a text search.

Is the PrimeTime® Gene Expression Master Mix compatible with different real-time PCR instruments or systems?

Yes. The master mix has been successfully used in qPCR experiments run on the QuantStudio™ 7 Flex (Thermo Fisher Scientific), 7900HT (Thermo Fisher Scientific), 7500 (Thermo Fisher Scientific), the CFX384™ (BioRad), and the LightCycler® 480 (Roche) instruments. All such experiments used the cycling conditions provided in the protocol, 
PrimeTime Gene Expression Master Mix.

Reference dye is provided in a separate tube, so you may add it to the master mix at the levels required by your real-time PCR instrument.