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What adapters should I use with the xGen Prism DNA Library Prep Kit?
The Ligation 1 and Ligation 2 Adapters provided in the xGen Prism DNA Library Prep Kit should be used for library construction. Since these adapters attach partial adapter sequences, library amplification using xGen Unique Dual Index Primer Pairs is required to incorporate sample indexing sequences and add the P5 and P7 sequences. Other adapters should not be used in place of the Ligation 1 and Ligation 2 Adapters with the xGen Prism DNA Library Prep Kit, since these adapters are highly modified to enhance ligation efficiency.