Frequently asked questions

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What is the concentration of the Alt-R Cas9 nucleases and nickases?

The Alt-R Cas9 nucleases and nickases are stored at concentration of 61 uM (10 mg/mL) in 25 mM Tris-Cl, 300 mM NaCl, 0.1 mM EDTA, 1 mM DTT, 50% glycerol (v/v), pH 7.4.

These proteins are greater than 95% pure, free of detectable DNase/RNases, absent bacterial DNA (below the limit of detection), and contain less than 2 EU/mL endotoxin.