Frequently asked questions

Our Scientific Applications Support team has assembled a list of frequently asked questions to help you find answers quickly. Filter using one or more categories to focus on specific topics, or use the search bar to perform a text search.

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What is the expected reduction in off-target effects (OTEs), when using Alt-R® S.p. HiFi Cas9 Nuclease 3NLS?

In many cases, Alt-R S.p. HiFi Cas9 Nuclease 3NLS exhibits a 10–20 fold reduction in editing at problematic off-target sites, when compared with the standard Alt-R S.p. Cas9 Nuclease 3NLS. The amount of reduction in off-target editing is highly variable and can differ based on multiple factors, including off-target site, ribonucleoprotein (RNP) dose, and cell type.