Frequently asked questions

Our Scientific Applications Support team has assembled a list of frequently asked questions to help you find answers quickly. Filter using one or more categories to focus on specific topics, or use the search bar to perform a text search.

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What is the on-target efficiency of the Alt-R® S.p. HiFi Cas9 Nuclease 3NLS?

The on-target efficiency of Alt-R S.p. HiFi Cas9 Nuclease 3NLS is typically 90–100% of the standard Alt-R S.p. Cas9 Nuclease 3NLS efficiency at a specific target site. The efficiency of both nucleases varies based upon the target site, cell type, and experimental conditions.