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When preparing the qPCR reaction mix, should I thaw or keep reagents (such as the PrimeTime® Gene Expression Master Mix) on ice?
It is good practice, but not necessary to thaw or keep reagents on ice. We see no difference in performance between reaction mixes prepared with the PrimeTime® Gene Expression Master Mix that were used immediately vs those kept at room temperature for up to 24 hours. PrimeTime Gene Expression Master Mix also shows exceptional temperature stability, even after a heat stress test (up to 8 hr at 55°C). Review the data.