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Frequently asked questions

Our Scientific Applications Support team has assembled a list of frequently asked questions to help you find answers quickly. Filter using one or more categories to focus on specific topics, or use the search bar to perform a text search.

Why should I consider purification of my oligos?

Purification removes truncated products and other synthesis impurities. Our experience has shown that purifying an oligonucleotide that will be used in demanding applications saves both time and money in the long run. We recommend considering additional purification for any oligonucleotide that is to be used for an application other than routine PCR or DNA sequencing. As a general rule, IDT also recommends that any oligonucleotide longer than 40 bases should receive further purification.