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Frequently asked questions (FAQs)

How do I determine the T<sub>m</sub> of my sequence?

Nov 17, 2016, 18:24 PM
IDT offers a free online calculator, the OligoAnalyzer tool, that will determine the Tm of your oligonucleotide sequence, for reaction conditions you specify. Access it at https://www.idtdna.com/calc/analyzer. Be sure to include the Mg2+ and dNTP concentrations you plan to use in your reaction, as this will affect the Tm for your oligo.
Tags :
  • melting temperature
  • oligo design
  • oligoanalyzer
Categories :
  • Custom DNA & RNA
  • DNA oligos
  • FAQs
  • RNA oligos
  • Ultramer DNA oligos
  • Ultramer RNA oligos
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