Frequently asked questions (FAQs)
How do I determine the T<sub>m</sub> of my sequence?
Nov 17, 2016, 18:24 PM
IDT offers a free online calculator, the OligoAnalyzer tool, that will determine the Tm
of your oligonucleotide sequence, for reaction conditions you specify. Access it at https://www.idtdna.com/calc/analyzer
. Be sure to include the Mg2+
and dNTP concentrations you plan to use in your reaction, as this will affect the Tm
for your oligo.
- melting temperature
- oligo design
- Custom DNA & RNA
- DNA oligos
- RNA oligos
- Ultramer DNA oligos
- Ultramer RNA oligos