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Frequently asked questions (FAQs)
How do I determine the T<sub>m</sub> of my sequence?
IDT offers a free online calculator, the OligoAnalyzer™ Tool, that will determine the Tm of your oligonucleotide sequence for reaction conditions you specify.
Be sure to include the Mg2+ and dNTP concentrations you plan to use in your reaction, as this will affect the Tm for your oligo.
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