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Frequently asked questions (FAQs)

Is there a modification you would recommend to block a ligation reaction at the 3' end of an oligo?

Nov 17, 2016, 19:00 PM
There are several modifications that can block 3' extension. Most 3' modifications will block extension during PCR.

The modifications most frequently used for this purpose are the 3'ddC, 3' Inverted dT, 3' C3 spacer, 3' amino, and 3' phosphorylation.
Tags :
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  • oligonucleotide
Categories :
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  • Custom DNA Oligos
  • Customer support
  • DNA oligos
  • FAQs
  • Oligo modifications
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