How can I check the Tm of my oligos?

Jul 28, 2013, 15:24 PM
The Tm of an oligo can be determined using the free, online OligoAnalyzer® program under the SciTools® drop down menu on the IDT website. Enter the sequence in the "Sequence" box and update the settings to correspond with your assay conditions.  Click "Analyze" and the melting temperature (Tm) will be displayed along with additional properties of your oligo.
  • SciTools Applications
  • Oligo Characteristics
  • Oligoanalyzer
  • Tm