How do I calculate the melting temperature of oligos in salt solutions other than NaCl?

As long as you know the concentration of the monovalent cations in your solution (Na+, K+, etc), you can use our free OligoAnalyzer® program to calculate the Tm of your oligo. There is a box to the right of where you enter the sequence where you can specify the concentration of the salt in your buffer.
  • Tm
  • Oligoanalyzer