CRISPR screening is an experimental approach used to find a small number of important genes or genetic sequences within a massive number of genetic sequences (e.g., within the entire genome). CRISPR screening can identify genes that influence drug resistance, drug sensitivity, and many other physiological effects.
CRISPR screening is an experimental method that uses CRISPR genome editing to identify a small number of genes (out of the whole genome) involved in a specific physiological effect.
Applications of CRISPR screening include identifying genes that cause a disease, that cause drug resistance or sensitivity in pathogenic organisms or in cancer cells, that cause susceptibility to environmental toxins, and many other uses in basic research on cells.
CRISPR screening works because of the ease with which insertion or deletion mutations (indels) can be introduced at any targeted site in the genome by CRISPR genome editing. These specific gene knockout events are the key for most CRISPR screening experiments .
Most CRISPR screening is done in cell culture, although some methods have been devised for use in animal models. In most CRISPR screening experiments, scientists knock out every gene that could possibly be important for a specific phenotype, knocking out only one gene per cell. In the resulting population of cells, some cells die, while others survive and may even be able to grow better, becoming the predominant cell type. Then, the scientists do next-generation sequencing (NGS) on the final population of cells to determine the gene responsible for the observed phenotype.
Some CRISPR screens use individually plated (arrayed) CRISPR RNA (crRNA), which can be used in ribonucleoproteins to perform CRISPR screening on cells in multiwell plates. This approach does not use lentiviruses. However, most CRISPR screens start with a pool of DNA oligos which are used to make a pool (library) of lentiviruses for the screen. Both the non-viral (arrayed) and viral (pooled) approaches to CRISPR screening have pros and cons. Selecting the type of screen should take into consideration many factors. For more detailed information on this choice and for more explanation of how both types of screens are performed and used, see our DECODED article, Overview: What is CRISPR screening?
RNA interference (RNAi) can also be used to screen the genome for genes of interest, but the RNAi approach is limited by its relatively low efficiency and specificity. CRISPR screens circumvent these limitations and can be used to target almost every gene in any genome systematically, to determine the roles genes play in specific physiological effects. However, RNAi screens still have value when scientists wish to screen for genes that have different effects when partially knocked down. The choice of method therefore depends on the kind of results needed in the experiment.