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Scientific Fundamentals Explained

Attach oligos to ligands or surfaces—Dithiol modifier

Dithiol Modifier Quick Facts
Availability: DNA or RNA
Location: 5’, 3’, or internally (shown)
Scales: 100 nmole to large scale
Purification: HPLC required
Symbol (for ordering through IDT): /5DTPA/, /iDTPA/, /3DTPA/

Thiol-modified oligonucleotides are used in attachment chemistry reactions to bind an oligo to a target. Targets are commonly gold, but can also include a variety of fluorescent and nonfluorescent moieties. The Dithiol modifier adds two functionalized thiol (SH) groups, increasing its target binding efficiency when compared to the conventional Thiol modifier. An additional Dithiol can be added for even greater binding efficiency.

Both Thiol- and Dithiol-modified oligonucleotides are shipped in their oxidized (disulfide) states and require reduction by Dithiothreitol (DTT) or Tris (2-carboxyethyl) phosphine (TCEP) prior to use.
To order, select Dithiol from the 5’ mods, Internal mods, or 3’ mods tab on the oligo order page. Please note this modification requires HPLC purification.

Disclaimer: Diethylene triamine pentaacetic acid (DTPA) and derived products (Chemical Products) are for research purposes only, and may not be used for commercial, clinical, diagnostic or any other use. These products or portions thereof are subject to proprietary rights of FRIZ Biochem Gesellschaft für Bioanalytik mbH. Patents Pending.
Author: Jeremy Pritchard is a Technical Support Representative at IDT.

© 2011 Integrated DNA Technologies. All rights reserved. Trademarks contained herein are the property of Integrated DNA Technologies, Inc. or their respective owners. MGB Eclipse is a registered trademark of Elitech Group. For specific trademark and licensing information, seewww.idtdna.com/trademarks.


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