In less than a decade, CRISPR genome editing has greatly changed the way scientists approach biological problems. With CRISPR, it is now possible to change (edit) the genomic DNA sequence of any living organism. However, the technology can appear overwhelming for researchers who are new to genome editing. We prepared this guide to help beginners learn both the theoretical and practical aspects of this revolutionary technology so that they can make this powerful tool a part of their research.
Overview of how The CRISPR basics handbook can help you
As explained in the handbook, CRISPR is easy to perform with what are now well-defined reagents: a CRISPR-associated (Cas) enzyme and a guide RNA (gRNA). The CRISPR basics handbook explains the functions of Cas enzymes and gRNA and describes how CRISPR works. Helpful explanations are given of non-homologous end-joining (NHEJ) and homology-directed recombination (HDR), both of which are used in CRISPR genome editing.
The handbook provides step-by-step instructions on conducting CRISPR experiments for different research goals. Topics include how to design guide RNAs, how to disrupt a gene using CRISPR, how to correct a gene mutation, and many CRISPR applications. There are descriptions of CRISPRa and CRISPRi, as well as a discussion of how CRISPR components can be used in studies of nuclear organization and epigenetic modifications. Detailed explanations of how to choose between different kinds of Cas enzymes (including CRISPR-Cas12a and variants of CRISPR-Cas9 such as Cas9 nickases and dCas9) are given.
Methods for delivery of CRISPR reagents to cells and appropriate use of controls are described as well. For researchers who need to use HDR in their CRISPR genome editing projects, details are given for how to design HDR donor templates and choose enhancers. Finally, the handbook goes into detail about analysis of CRISPR-treated samples: how to determine if the desired edit has been accomplished, as well as how to analyze off-target effects.
Get this helpful resourceThe entire handbook is designed to be approachable, to help researchers understand the “why and how” of each step in CRISPR genome editing from experimental conception to data analysis, as well as to understand practical applications of CRISPR technology.
Download your copy of The CRISPR basics handbook.