Positive control crRNAs can be used to show that Cas12a editing is occurring in your experiments, which can be useful when
you are optimizing ribonucleoprotein (RNP) delivery conditions or if you need to troubleshoot your experiments.
Attention: In addition to using positive controls, we suggest testing 3 or more crRNAs per target gene.
Our scientists have designed and tested positive control crRNAs targeting HPRT (Figure 4). To order, copy and paste the appropriate
sequence in the Cpf1 crRNA ordering page:
Human HPRT1, Cas12a Positive Control crRNA: GGTTAAAGATGGTTAAATGAT
Mouse Hprt, Cas12a Positive Control crRNA: GGATGTTAAGAGTCCCTATCT
Rat Hprt1, Cas12a Positive Control crRNA: ACCGCCCCCCCCATACCCCAA
Our scientists have also designed and tested PCR primers (Alt-R HPRT PCR Primer Mixes for human, mouse, or rat) for use
with the Alt-R Genome Editing Detection Kit to detect editing or estimate editing efficiency in samples transfected
with the positive control HPRT crRNAs.