Frequently asked questions

Our Scientific Applications Support team has assembled a list of frequently asked questions to help you find answers quickly. Filter using one or more categories to focus on specific topics, or use the search bar to perform a text search.

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How much Alt-R® Cpf1 Electroporation Enhancer should I use?

We have observed that dose response curves vary for different guide sequences, depending on potency of the guide. We recommend titrating the amount of RNP and keeping the amount of enhancer fixed.

For the Amaxa® Nucleofector® System (Lonza), we recommend 4 µM of Alt-R® Cpf1 Electroporation Enhancer, and for the Neon®System (Thermo Fisher Scientific), we recommend 1.8 µM of Alt-R Cpf1 Electroporation Enhancer. Toxicity may be observed at high concentrations of enhancer.