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Frequently asked questions

Our Scientific Applications Support team has assembled a list of frequently asked questions to help you find answers quickly. Filter using one or more categories to focus on specific topics, or use the search bar to perform a text search.

How much Alt-R™ Cas12a Electroporation Enhancer should I use?

We have observed that dose response curves vary for different guide sequences, depending on potency of the guide RNA. We recommend titrating the amount of ribonucleoprotein (RNP) and keeping the amount of electroporation enhancer fixed.

For the Nucleofector™ System (Lonza), we recommend 3 µM of Alt-R Cas12a Electroporation Enhancer, and for the Neon™ System (Thermo Fisher Scientific), we recommend 1.8 µM of Alt-R Cas12a Electroporation Enhancer. Toxicity may be observed at high concentrations of enhancer.

*RUO—For research use only. Not for use in diagnostic procedures. Unless otherwise agreed to in writing, IDT does not intend for these products to be used in clinical applications and does not warrant their fitness or suitability for any clinical diagnostic use. Purchaser is solely responsible for all decisions regarding the use of these products and any associated regulatory or legal obligations.