Frequently asked questions

Our Scientific Applications Support team has assembled a list of frequently asked questions to help you find answers quickly. Filter using one or more categories to focus on specific topics, or use the search bar to perform a text search.

FAQ categories

CRISPR genome editing 
Custom DNA & RNA 
Functional genomics 
Genes & gene fragments 
Next generation sequencing 
Optional services 
qPCR & PCR 
Reagents & kits 

Search all FAQs:

I cannot see CRISPR-Cpf1 editing. Why?

Ensure that you have included the Alt-R™ Cpf1 Electroporation Enhancer (carrier DNA) in the electroporation and that you are using one of our positive control crRNAs to demonstrate efficient delivery.

Verify that the target site in your cells does not show any polymorphism that could affect the potency of the crRNA.

Target 3 or more PAM sites in your gene of interest to identify a site that provides optimal editing efficiency, because we have found that approximately 50% of the Cpf1 PAM sites give detectable editing.


Categories:
CRISPR genome editing

Tags:
crispr alt-r Cpf1 electroporation