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Frequently asked questions
Our Scientific Applications Support team has assembled a list of frequently asked questions to help you find answers quickly. Filter using one or more categories to focus on specific topics, or use the search bar to perform a text search.
I cannot see CRISPR-Cpf1 editing. Why?
Ensure that you have included the Alt-R™ Cpf1 Electroporation Enhancer (carrier DNA) in the electroporation and that you are using one of our positive control crRNAs to demonstrate efficient delivery.
Verify that the target site in your cells does not show any polymorphism that could affect the potency of the crRNA.
Target 3 or more PAM sites in your gene of interest to identify a site that provides optimal editing efficiency, because we have found that approximately 50% of the Cpf1 PAM sites give detectable editing.