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Frequently asked questions
Our Scientific Applications Support team has assembled a list of frequently asked questions to help you find answers quickly. Filter using one or more categories to focus on specific topics, or use the search bar to perform a text search.
Why can't I see CRISPR-Cas12a (Cpf1) editing?
Ensure that you have included the Alt-R™ Cpf1 Electroporation Enhancer (carrier DNA) in the electroporation and that you are using one of our positive control crRNAs to demonstrate efficient delivery.
Verify that the target site in your cells does not show any polymorphism that could affect the potency of the crRNA.
Target additional PAM sites in your gene of interest to identify a site that provides optimal editing efficiency.
Categories:
CRISPR genome editing*RUO—For research use only. Not for use in diagnostic procedures. Unless otherwise agreed to in writing, IDT does not intend for these products to be used in clinical applications and does not warrant their fitness or suitability for any clinical diagnostic use. Purchaser is solely responsible for all decisions regarding the use of these products and any associated regulatory or legal obligations.