No, CRISPR-Cas9 complexes do not recognize, or recognize extremely poorly, targets lacking PAM sequences .
The PAM sequence recognized by the S. pyogenes CRISPR-Cas9 system is NGG. If this sequence is not present in your target, you may be able to use other CRISPR systems (from other bacterial species) that recognize different PAM sequences. The following table lists examples of PAM sequences:
|Bacterial species||PAM sequence||Reference|
|S. pyogenes (Cas9)*||NGG|||
|Acidaminococcus sp BV3L6 (Cpf1)*||TTTV|||
|Streptococcus thermophilus|| NNAGAA
* In addition to the Alt-R® CRISPR-Cas9 System, we offer the Alt-R CRISPR-Cpf1 System (crRNA, nuclease, and electroporation enhancer). Note that each CRISPR nuclease has unique requirements for crRNA and electroporation enhancer design. You will not be able to use Cas9 and Cpf1 reagents in the same reaction.
*RUO—For research use only. Not for use in diagnostic procedures. Unless otherwise agreed to in writing, IDT does not intend for these products to be used in clinical applications and does not warrant their fitness or suitability for any clinical diagnostic use. Purchaser is solely responsible for all decisions regarding the use of these products and any associated regulatory or legal obligations.