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Frequently asked questions

Our Scientific Applications Support team has assembled a list of frequently asked questions to help you find answers quickly. Filter using one or more categories to focus on specific topics, or use the search bar to perform a text search.

Is it possible for the Alt-R™ Cas12a Electroporation Enhancer to integrate at the Cas12a cut site?

Our internal research data, utilizing next generation sequencing (NGS), showed a very low rate of integration of the enhancer into the target site (fewer than 1 in 10,000 reads, which is much lower than the insertion rate of random pieces of cellular DNA). Therefore, enhancer integration should not negatively impact experimental outcomes.



*RUO—For research use only. Not for use in diagnostic procedures. Unless otherwise agreed to in writing, IDT does not intend for these products to be used in clinical applications and does not warrant their fitness or suitability for any clinical diagnostic use. Purchaser is solely responsible for all decisions regarding the use of these products and any associated regulatory or legal obligations.