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Frequently asked questions

Our Scientific Applications Support team has assembled a list of frequently asked questions to help you find answers quickly. Filter using one or more categories to focus on specific topics, or use the search bar to perform a text search.

When using the Alt-R® Cas9 nickases, do I follow the same protocol as the standard Alt-R S.p. Cas9 Nuclease 3NLS?

Yes. The delivery of the CRISPR-Cas9 ribonucleoprotein (RNP) complexes with Alt-R Cas9 nickases is similar to RNP with the standard Alt-R S.p. Cas9 Nuclease 3NLS, so the same protocol can be used with these enzymes. However, with the Cas9 nickases, two crRNAs are required to generate a double-strand break, while only one crRNA is required for the standard nuclease.

When preparing materials to deliver two crRNAs simultaneously, first prepare RNPs with each crRNA separately before RNP delivery. This will help prevent undesireable hybridization between RNAs.

*RUO—For research use only. Not for use in diagnostic procedures. Unless otherwise agreed to in writing, IDT does not intend for these products to be used in clinical applications and does not warrant their fitness or suitability for any clinical diagnostic use. Purchaser is solely responsible for all decisions regarding the use of these products and any associated regulatory or legal obligations.