Functional genomics: Loss-of-function studies

For knockdown of mRNA, lncRNA, and miRNA

IDT offers 3 options that are optimized to provide increased potency and specificity in loss-of-function studies that involve transfection of short oligos:

  • Dicer-substrate short interfering RNAs (DsiRNAs)
    RNA interference (RNAi) is a conserved, gene regulation mechanism that can be used to knock down expression of RNA in most eukaryotic cells. For example, use DsiRNAs to decrease mRNA or noncoding RNA expression via Dicer and the RNA-induced silencing complex (RISC) pathway. Note: Dicer localizes to the cytoplasm, so this method is most effective when targeting RNA in the cytoplasm.
  • Antisense oligonucleotides (ASOs)
    Although ASOs are most potent in the nucleus, ASOs can be used to target mRNA and long noncoding RNA (lncRNA) in both the nucleus and cytoplasm. Binding of an ASO to RNA triggers degradation of the target RNA by RNase H.
  • MicroRNA (miRNA) inhibitors
    IDT miRNA Inhibitors are steric blocking oligonucleotides that hybridize to mature miRNAs, inhibiting their function.

For information about gene knockout experiments using CRISPR-Cas9 genome editing, visit

DsiRNAs and TriFECTa® Kits

Dicer-substrate siRNAs (DsiRNAs) are chemically synthesized, 27mer duplex RNAs that show increased potency in RNAi compared to traditional 21mer siRNAs.

Choose from Predesigned, Custom, and Control DsiRNAs, or select a TriFECTa® Kit (Predesigned DsiRNAs, Control DsiRNAs, and buffer in a convenient kit).

DsiRNAs/TriFECTa Kits ≫

Antisense Oligonucleotides

Antisense oligonucleotides (ASOs) are short, synthetic oligonucleotides (15–25 nt) that include phosphorothioate linkages and modified bases, which confer nuclease resistance.

Antisense Oligonucleotides ≫

IDT® miRNA Inhibitors

IDT miRNA inhibitors contain 2′-O-methyl residues that confer resistance to endonuclease degradation and increase binding affinity to RNA targets, while ZEN™ modifications block exonuclease degradation.

miRNA Inhibitors ≫