Frequently asked questions

Our Scientific Applications Support team has assembled a list of frequently asked questions to help you find answers quickly. Filter using one or more categories to focus on specific topics, or use the search bar to perform a text search.

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How many freeze-thaw events can PrimeTime® Gene Expression Master Mix withstand?

We tested up to 20 freeze-thaw cycles, which is more extreme than typical laboratory usage. No detrimental effects or loss in Cq were observed. However, we still recommend following good laboratory practice by minimizing freeze-thaw cycles. Download the white paper, Ambient shipping of PrimeTime Gene Expression Master Mix, for more data.


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qPCR & PCR