Frequently asked questions

Our Scientific Applications Support team has assembled a list of frequently asked questions to help you find answers quickly. Filter using one or more categories to focus on specific topics, or use the search bar to perform a text search.

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How much Alt-R® Cas9 Electroporation Enhancer should I use during delivery of the ribonucleoprotein (RNP) complex?

We see dose response curves that vary for different guide sequences, depending on the potency of the guide sequence. We recommend titrating the amount of RNP for each cell type of interest.

Start with a 1:1 ratio of enhancer:RNP and optimize using a titration gradient of the RNP. For the Amaxa® Nucleofector® System (Lonza), we recommend 4 µM of Alt-R® Cas9 Electroporation Enhancer, and for the Neon® System (Thermo Fisher Scientific), we recommend 1.8 µM of Alt-R Cas9 Electroporation Enhancer.