Frequently asked questions

Our Scientific Applications Support team has assembled a list of frequently asked questions to help you find answers quickly. Filter using one or more categories to focus on specific topics, or use the search bar to perform a text search.

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I cannot see CRISPR-Cas9 editing. What can I do?

  • Double-check that the your crRNA sequence matches the species and target location as expected. For example, verify that the target site in your cells does not show any polymorphism that could affect the potency of the crRNA.
  • Confirm that you can observe successful editing using a known positive control guide RNA, such as the Alt-R® positive control crRNAs for human, mouse, or rat.
  • If the positive controls work but the experimental crRNA does not, test additional target sites nearby to see if a more efficient guide RNA can be found.
For additional support, contact our experts at applicationsupport@idtdna.com.