Frequently asked questions

Our Scientific Applications Support team has assembled a list of frequently asked questions to help you find answers quickly. Filter using one or more categories to focus on specific topics, or use the search bar to perform a text search.

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Should I avoid purification for sequences containing mixed bases (degenerate or randomized oligos)?

The decision to order purified oligos will vary by your research needs. Purification involves collecting fractions of the total product synthesized and will inherently reduce variability in the oligos, possibly skewing the random base ratios, resulting in less overall randomness. Taking this into consideration, there are some cases where purification may still be warranted, especially with very long or highly modified oligos. If you have additional questions regarding purification of your mixed base oligos, contact customer service at custcare@idtdna.com.