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Top Questions

How can I tell if my oligos successfully annealed?

The hybridized oligos or DNA duplex can be run on a non-denaturing gel wtih appropriate molecular weight markers. Alternatively, run the duplexed oligo side by side with single-stranded oligo. If annealing was successful, the double-stranded band will be shifted up from the single-stranded band. Use UV backshadowing or a stain such as SYBR® Gold to visualize the bands. Ethidium Bromide may not sufficiently stain the single-stranded DNA to make it visible.
Tags:
  • anneal
  • Duplex
  • gel electrophoresis
  • single-stranded
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