Top Questions

Is the PrimeTime® Gene Expression Master Mix compatible with different real-time PCR instruments or systems?

Yes. The PrimeTime® Gene Expression Master Mix has been successfully used in qPCR experiments run on the QuantStudio™ 7 Flex (Thermo Fisher Scientific), 7900HT (Thermo Fisher Scientific), 7500 (Thermo Fisher Scientific), the CFX384™ (BioRad), and the LightCycler® 480 (Roche) instruments, using the cycling conditions provided in the PrimeTime® Gene Expression Master Mix protocol.

Reference dye is provided in a separate tube, so you may add it to the master mix at the levels required by your real-time PCR instrument.

Tags:
  • qPCR
  • qPCR master mix
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