TechVault

What quality checks do gBlocks Gene Fragments with variable bases receive?

The constant regions are gBlocks Gene Fragments and undergo size verification by capillary electrophoresis and sequence verification by mass spectrometry. The variable regions cannot currently receive complete analysis (using NGS, which would be needed for this analysis, would be cost prohibitive). We rely on a validated process that we have experimentally confirmed by NGS that ensures >80% of DNA species are present in the final 200 ng of material shipped.
Tags:
  • variable
  • mutagenesis
  • gBlocks
  • gblock
  • libraries