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Top Questions

In what direction does IDT synthesize oligos?

We synthesize our oligos in the 3’ -> 5’ direction off of a controlled-pore glass (CPG) solid support using amidite chemistry. This 3’ -> 5’ technique mitigates the effect of truncated product on primer binding, as it will be limited to the 5’ end.
Tags:
  • Oligo Synthesis
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