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IDT® miRNA Inhibitors


IDT® miRNA Inhibitors are highly potent and specific microRNA (miRNA) inhibitors designed for functional analysis of miRNAs. They are short, single stranded oligonucleotides comprised of 2’-O-methyl residues with additional ZEN modifications. Incorporating 2’-O-methyl residues confers resistance to endonuclease degradation and increases binding affinity to RNA targets, while the ZEN modification blocks exonuclease degradation and further increases binding affinity.

Find mature miRNA sequences in the miRNA database, miRBase [1-4], and then copy and paste them into the sequence box below.

Sequence Entry




Step 1

Select the required product size (default is 5 nmol). [Required]

Step 2

Provide a unique name for your sequence. This name will appear on the product label. [Required]

Step 3

Go to the miRNA database, miRBase [1-4]. Search for your miRNA of interest. You may use the sequencing data in miRBase to help select the appropriate strand to target.

Step 4

Copy the mature sequence and paste into the Sequence box of the IDT® miRNA Inhibitor ordering tool. Keep the sequence format provided in miRBase. The mature miRNA sequence will be automatically converted to an IDT® miRNA Inhibitor when you click Add to Order. NOTE: The IDT® miRNA Inhibitor sequence will be one nucleotide shorter than the corresponding miRNA sequence.

Step 5

Enter any note or comments about the sequence. This is information for you. IDT does not use this information for manufacturing or ordering purposes.

Step 6

Click Add to Order. You will be taken to the Shopping Cart, where you can review your order.

Entry Number:

Choose a Product:



Name


Sequence*

Bases

Notes


*The IDT® miRNA Inhibitor sequence will be one nucleotide shorter than the corresponding miRNA sequence.

Step 1

Create a document using a text editor (e.g., Notepad) from which to paste your sequences. Please do not use a word processor such as Microsoft Word, which may add hidden characters and formatting to your entries and cause the order to fail. You can also paste from a Microsoft Excel spreadsheet.

Step 2

Create your entries in the text editor in one of the following formats, using a new line for each entry:
a. Name Sequence Notes (using a tab to separate each item in the entry)
b. Name, Sequence, Notes (using a comma to separate each item in the entry)
c. Name; Sequence; Notes (using a semicolon to separate each item in the entry)

Step 3

Go to the miRNA database, miRBase [1-4], search for your miRNA of interest. You may use the sequencing data in miRBase to help select the appropriate strand to target.

Step 4

In the text editor (using one of the formats indicated in Step 2):
a. Provide a unique name for your sequence. This name will appear on the product label. [Required]
b. Copy the mature sequence you are interested in and paste next to the name. Keep the sequence format provided in miRBase. The mature miRNA sequence will be automatically converted to an IDT® miRNA Inhibitor when you click Add to Order.
NOTE: The IDT® miRNA Inhibitor sequence will be one nucleotide shorter than the corresponding miRNA sequence.

c. Enter any notes or comments about the sequence. This is information for you. IDT does not use this information for manufacturing or ordering purposes. Remember not to add punctuation to your notes which could affect the format above.

Step 5

Repeat steps 4–5 until all of your desired miRNA sequences have been added to your document.

Step 6

In the miRNA Inhibitor Tool:
a.Select the scale and purification for your miRNA Inhibitor (if you need more than one scale and purification, you can change these individually by clicking Review Order).


b. Copy the text from your document and paste into the text box provided.

c. Click Review Order to review your entries and choose different scales

or click Add to Order to be taken directly to the Shopping Cart. Your order can also be reviewed in the Shopping Cart.

Choose a Product:





*The IDT® miRNA Inhibitor sequence will be one nucleotide shorter than the corresponding miRNA sequence.


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