PrimeTime® qPCR Application Guide

Experimental Overview, Protocol, and Troubleshooting
4th Edition (2015)

The qPCR Application Guide provides a general overview, guidance, and troubleshooting advice on the entire qPCR process, from RNA isolation to data analysis.

You can use the guide to:

  • Review the latest recommendations for qPCR protocols: experimental setup, performance, and analysis
  • Learn specific information regarding the 5′ nuclease assay, including specific resuspension instructions
  • Troubleshoot common problems


qPCR Guide 2012 Cover
What's New in the 4th Edition:
  • Updated instrument-dye compatibility information
  • Updated representative data about master mixes