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Custom labels, RxnReady, Tubes, Plates, Degenerate Bases, Custom Normalization: Many choices, made easy

Single Entry
Single Entry Enter modified sequences and more complex structures,
one at a time.
Multiple Entry
Multiple Entry Number of Items:   
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RxnReady
Enter modified sequences and more complex structures,
one at a time.

Excel or Text Paste Entry
Excel or Text Paste Entry Paste multiple sequences from Excel or a text file directly into the website
Duplex Entry
Duplex Entry Enter duplexed DNA, one duplex at a time.

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Desalted custom synthesized DNA oligos are shipped lyophilized or customized according to your specifications. Synthesis scales up to 1 µmole are shipped the next business day. 5 µmole and 10 µmole scales are shipped within 5 business days.

Base Pricing

  • No setup fee for standard DNA synthesis
  • Large-scale synthesis (up to 10 grams) available. Email custcare@idtdna.com
ProductPricingLength
25 nmole DNA Oligo$0.35 USD / Base15 - 60 BasesOrder
100 nmole DNA oligo$0.55 USD / Base10 - 90 BasesOrder
250 nmole DNA oligo$0.95 USD / Base5 - 100 BasesOrder
1 µmole DNA oligo$1.95 USD / Base5 - 100 BasesOrder
5 µmole DNA oligo$9.50 USD / Base5 - 50 BasesOrder
10 µmole DNA oligo$17.50 USD / Base5 - 50 BasesOrder

Yield Guarantees for Standard Desalted DNA Oligos

At no extra charge, every oligo is deprotected and desalted to remove small molecule impurities. Oligos are quantitated twice by UV spectrophotometry to provide an accurate measure of yield. Mass yield, as measured in Optical density (OD), varies with length and decreases for shorter oligos. All oligos are quality control (QC) checked by mass spectrometry.

For more information on yields, please see the Technical Report Oligonucleotide Yield Resuspension and Storage.

Synthesis Scale Oligo Length Yield Guarantee
25 nmole 15–60 Bases 3 ODs
100 nmole 10–90 Bases 6 ODs
250 nmole  5–100 Bases 15 ODs
1 µmole
 5–100 Bases
 45 ODs
5 µmole
 5–50 Bases
 225 ODs
10 µmole
 5–50 Bases
 450 ODs

DNA Synthesis

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Lab Ready
$2.50 USD
Mixing
$10.00 USD
Mixed Bases
$125.00 USD
Custom Normalized Tube
$5.00 USD

LabReady Oligo Service

IDT will rehydrate your oligos at a fixed concentration prior to shipment as part of our LabReady Oligo Service. For a fee, your 25, 100, or 250 nmole scale oligos are shipped hydrated at 100 µM in IDTE (1X TE Buffer), pH 8.0.

Custom Normalized Tubes

For ultimate convenience, specify the concentration at which you would like to receive your oligos ($5.00 per oligo). Buffer choices are RNase-free water*, or IDTE (1X TE Buffer), pH 8.0 or 7.5. Applies to 25 nmole, 100 nmole, 250 nmole, and 1 µmole scales. For resuspension of larger scales, please contact customer service at custcare@idtdna.com.

RxnReady

For a mixing fee, have 2–6 standard desalt oligos premixed in a single tube according to your specifications. Oligos may contain modifications. Yields range between 5-50 nmole, and oligos can be shipped lyophilized or hydrated (in RNase-free water* or IDTE 8.0).

* To ensure longer stability of oligos, IDT recommends resuspension in TE. Learn more at DNA Oligonucleotide Resuspension and Storage

Mixed Bases

IDT offers oligonucleotides containing randomized, or “mixed bases”. Mixed Bases are used in primers to bind to templates that contain variability or a mixture of sequences at the primer binding site. Mixed Bases can also be used to create diversity in clone libraries and in site-directed mutagenesis.

IDT offers two types of randomization, Machine Mixed and Hand Mixed Bases. Machine Mixed Bases can be made at any/all base sites at no additional charge. At these base positions, the synthesizer pulls an equal ratio of the desired bases, but their different coupling rates do not guarantee an equal ratio of incorporation. 

Hand Mixed Bases provide customer-defined ratios of the desired bases, where coupling efficiencies are taken into account. For each mixture, IDT can incorporate up to 100 residues on the 100 nmole and 250 nmole scales and up to 40 residues on the 1 µmole scale, with a maximum of 4 unique Hand Mixes per oligonucleotide.When entering your sequence, use the “Mixed Base” tab at the bottom of the page. It lists the IUPAC-IUB symbols and is where custom mix ratios should be entered.

Additional Customized Formulation and Mixing Services

For aliquots or for more complex formulation or mixing needs, please contact customer service at custcare@idtdna.com.

Formulation and Mixing Services

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Annealing Services

Have complementary oligos annealed prior to shipment. Annealed oligos are supplied dry.

Description100 nmole Duplex250 nmole Duplex1 umole Duplex
Annealing Fee$30.00 USD$40.00 USD$45.00 USD

Other Custom Preparative Services

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Customizing labels to fit your needs has never been easier than with our online label designer. Simply select from a list of available fields and drag them onto your label template. You can include:

  • Sales Information (Purchase Order #, Name, University)
  • Manufacturing Data (Date, Product Name, Sequence)
  • Product Specifications (OD, # Bases, Molecular Weight, TM)
  • Lab Specific Info (Custom notes from your lab, include your own image or logo
IDT has integrated the printing of custom labels directly into its manufacturing system, thereby removing the need for the labels to be printed and tracked manually. Create as many designs as you like and easily assign them to your tubes with a simple dropdown list. Uploading your own images will enable you to easily sort and identify your tubes in the freezer or upon receipt.

For a quick tutorial, click here.

To create a label template, click here.

The custom label option is also available at the bottom of the page when you order your oligos.

Order Oligos

Custom Labels

DNA Oligonucleotide Resuspension and Storage

IDT recommends resuspension in TE buffer rather than water. Oligonucleotides resuspended in water will have less stability and will have to be shipped on dry ice, which is more costly than standard shipping. Stability studies at IDT have shown that oligonucleotides stored in water at room temperature may show some degradation in as little as 60 days. The same studies showed that oligonucleotides stored dry or in TE at room temperature did not show signs of degradation for at least 7 months. More Info ▶

Starting Scale and Yield

IDT uses a starting scale, referred to as just “scale”, for the amount of initiating nucleotide used to begin oligo synthesis. However, processing steps such as cleavage and deprotection of the oligonucleotide will reduce the final delivery amount (yield). The addition of modifications, purification and length combinations also all affect the final yield of each oligo, which will always be less than the starting scale. The minimum yield guarantee is given in the shopping cart and in the order confirmation email. To receive more final yield, select a larger synthesis scale. More Info ▶

Purification and Yield

Additional purification significantly improves oligonucleotide performance in demanding applications such as multiplex PCR, cloning, mutagenesis, and antisense/RNAi methods. However, purification will always lower oligonucleotide final yield. HPLC routinely provides 85% purity, while PAGE provides 90% purity. The final yield of a PAGE purified oligo, however, will be less than an HPLC purified oligo. For example, a 250 nmole scale 25mer oligo will yield 8X with standard desalt, but only 2X with HPLC purification and 1X with Page purification. More Info ▶

Tools for Oligonucleotide Analysis and Design

IDT provides a number of free oligonucleotide design and analysis tools in the online SciTools® suite. The suite is comprised of tools for designing assay components as well as determining the properties of any sequence entered. Check out the SciTools Suite website at www.idtdna.com/scitools

IDT Guarantees Quality

IDT has pioneered the use of high throughput Quality Control methods and is the only oligonucleotide manufacturer that offers 100% QC and purity guarantees. Today, every oligo synthesized at IDT undergoes QC by mass spectrometry. IDT also offers CE and analytical HPLC as routine tools for additional QC on purified oligos. In addition to DNA, IDT has led the effort over the last decade to reduce the cost of high-quality custom RNA synthesis. Through improvements to the traditional tert-butyldimethylsilyl (tBDMS) chemistry and advances in instrumentation, IDT has achieved the highest coupling efficiency in the industry. Only at IDT are customers guaranteed the very best in quality synthesis with free mass spectrometry and CE traces on all purified oligos.
 
 
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