Frequently asked questions

Our Scientific Applications Support team has assembled a list of frequently asked questions to help you find answers quickly. Filter using one or more categories to focus on specific topics, or use the search bar to perform a text search.

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How do I input the include/exclude regions on the PrimerQuest "Advanced" menu?

Only one included region may be defined per design in the PrimerQuest design tool. The included region is entered as a range; for example, 1200-1500. Multiple excluded regions are allowed per design in PrimerQuest. The "Excluded Region List" is a comma separated list of ranges. For example, 1200-1500, 1600-1700, 1850-1950. Find both of these parameter fields on the main page of the PrimerQuest design tool http://www.idtdna.com/Primerquest/Home/Index under the "Custom Target Region" section. Read more about how to use the PrimerQuest tool in PrimerQuest® Design Tool: From Basic to Highly Customizable Designs.

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qPCR & PCR