Top Questions

What methods can be used to deliver the Cpf1 ribonucleoprotein (RNP)?

We have successfully used electroporation to deliver the Cpf1 RNP complex. Alt-R® Cpf1 Electroporation Enhancer, a non-targeting carrier DNA, is also required for efficient delivery of the RNP complex. Although less efficient than electroporation, we also have successfully delivered the Cpf1 RNP into HEK-293 cells via lipofection using RNAiMAX™ reagent (Thermo Fisher Scientific).

Tags:
  • Cpf1
  • CRISPR
  • Alt-R
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