Click chemistry is a two-step process that uses quantitative chemical reactions of alkyne and azide moieties to create covalent carbon-heteroatom bonds between biochemical species.1 The reaction uses copper(I) as a catalyst and forms a 1,2,3-triazole between an azide and terminal alkyne.2 The technology is reliable and stable which makes it an ideal oligonucleotide labeling method.
The benefits of click chemistry include1:
|Modification Name||Used for modifying oligos with|
|5', Int, 3' Azide (NHS Ester)||5’, 3’, or internal azide functional group|
|5' Hexynyl||5’ alkyne functional group|
|5', Int, 3' 5-Octadiynyl dU||5’, 3’, or internal alkyne functional group|
|5', Int Biotin (Azide)||5’ or internal biotin functional group|
|5', Int 6-FAM (Azide)||5’ or internal 6-FAM functional group|
|5', Int 5-TAMRA (Azide)||5’ or internal 5-TAMRA functional group|
To order an alkyne or azide modified oligo:
Specify the nucleic-acid sequence along with the appropriate alkyne or azide modifications included in the sequence.
To order an oligo modified by a 1,2,3-triazole linkage of Biotin, 6-FAM, or 5-TAMRA :
Select the desired azide modification at the desired location within the nucleic-acid sequence. IDT will synthesize the oligo, perform the click chemistry reaction, and purify the product.
Click-Chemistry enabled, modified oligos are manufactured and sold under license from Baseclick GmbH, using Base-click's proprietary Click Chemistry. All such modified products are sold by IDT for the end-user's internal research purposes only. See www.baseclick.org for further details.