xGen SARS-CoV-2 Amplicon Panel enables researchers to identify SARS-CoV-2 strains, including variants, by creating next generation sequencing (NGS) libraries. The sequencing data can be used to answer research questions such as:
- Which viral strains account for most infections within a local population?
- What new strains are emerging?
IDT’s predesigned amplicon panels for COVID research can aid in identifying the extent and pattern of viral spread within a population and allow researchers to quantify the effectiveness of intervention measures.
Workflow of the IDT SARS-CoV-2 Panel
After isolating the genomic material from a research sample and then converting the SARS-CoV-2 RNA into cDNA, the xGen SARS-CoV-2 Amplicon Panel can be used to create an NGS library. The workflow consists of a 2-step PCR amplification that occurs in a single tube
(Figure 1). The first PCR reaction amplifies the SARS-CoV-2 cDNA into 345 overlapping amplicons. The second, indexing PCR adds the adapter/index to each end. For research applications where more than one library is multiplexed for sequencing, IDT
offers the xGen Normalase™ reagent that can normalize the libraries so each is represented in the final sequence data.