DNA methylation is the most widely studied epigenetic modification. DNA methylation occurs primarily on cytosine bases in the CpG dinucleotide motif in most mammalian cells, and across all cytosine contexts in plant genomes. DNA methylation is often associated
with the regulation of gene expression through its presence or absence in CpG islands among gene promoter regions, and these methylation patterns can be used to identify different cell types and disease states .
IDT offers two products for methylation sequencing:
- xGen Methyl-Seq DNA Library Prep Kit for whole genome and targeted methylation sequencing
- xGen Adaptase Module for single cell methylation sequencing
Some common applications of methyl-seq include:
- Whole genome bisulfite sequencing (WGBS) research
- Identifying genome-wide methylation of cell-free (cfDNA) in research studies
- Hybridization capture for targeted methylation studies
- Bisulfite-converted DNA enriched by MeDIO, chromatin immunoprecipitation (ChIP), or other research methods
xGen Methyl-Seq Library Prep Kits
The xGen Methyl-Seq DNA Library Prep Kit can be used for WGBS and targeted bisulfite sequencing with the addition of an xGen Custom Hyb Panel.
The kit’s post-bisulfite library preparation workflow offers an efficient library preparation method. The kit is compatible with low input quantities such as cfDNA for biomarker research studies. The xGen Methyl-Seq DNA Library Prep Kit is also
compatible with bisulfite-converted DNA samples enriched by ChIP or other methods as well as ancient DNA samples that may be deaminated.
2-hour workflow for bisulfite-converted samples
The xGen Methyl-Seq DNA Library Prep Kit workflow maximizes DNA recovery through a post-bisulfite library preparation, utilizing an efficient adapter attachment that is compatible with single-stranded, bisulfite-converted DNA (Figure 1). Library complexity
from this kit is significantly greater than those from methods that bisulfite convert after library construction (see Table 2).
Additionally, the template-independent adapter attachment chemistry of the xGen Methyl-Seq DNA Library Prep Kit provides a more complete, less biased library as observed from comprehensive methylome coverage by WGBS (see Table 2).