Shorter probes with high Tm and increased specificity compared to traditional 5′ nuclease probes
These locked nucleic acid probes are commonly used for genotyping and custom applications such as transcript variant detection or differential detection of microbial species. A
PrimeTime Mini LNA qPCR Probes are ideal for screening a small sample set or performing a few reactions to optimize probe designs.
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PrimeTime LNA qPCR Probes are offered with a wider selection of dyes and quenchers than PrimeTime Mini LNA qPCR Probes, and are best-suited for large-scale or high-throughput applications.
Locked nucleic acid nucleotides can be incorporated into effective qPCR probes[1–4]. Because these bases significantly increase Tm, PrimeTime LNA qPCR Probes can be designed with shorter lengths than standard qPCR probes. Shorter probes have better quenching and a higher signal-to-noise ratio and are, therefore, more sensitive. More importantly, these probes offer an improved ability to distinguish mutations or single nucleotide polymorphisms (SNPs) [1]. A locked nucleic acid probe can be designed to have a ΔTm of >15°C, which greatly increases
For design strategies and tips, see the Product details tab of the Affinity Plus qPCR Probes product page or contact applicationsupport@idtdna.com. Design fees may apply.
PrimeTime LNA qPCR Probes can be ordered either at a guaranteed yield of 0.5 nmol or at defined synthesis scales to suit your research needs: