LNA bases can be incorporated into effective qPCR probes[1–4]. Because LNA bases significantly increase Tm, PrimeTime LNA qPCR Probes can be designed with shorter lengths than standard qPCR probes. Shorter probes have better quenching and a higher signal-to-noise ratio and are, therefore, more sensitive. More importantly, these probes offer an improved ability to distinguish mutations or single nucleotide polymorphisms (SNPs) [1]. An LNA probe can be designed to have a ΔTm of >15°C, which greatly increases accuracy of allele determination in real-time PCR or other methods that use differential hybridization to distinguish polymorphisms.
For design strategies and tips, see the Product details tab of the Affinity Plus qPCR Probes product page or contact applicationsupport@idtdna.com. Design fees may apply.
PrimeTime LNA qPCR Probes can be ordered either at a guaranteed yield of 0.5 nmol or at defined synthesis scales to suit your research needs:
PrimeTime Mini LNA qPCR Probes
- Ideal for analyzing a small sample set or performing a few reactions to optimize probe designs
- Available with FAM, HEX™ or YAK® fluorescent dyes, and Iowa Black® FQ quencher
- Guaranteed normalized yield of 0.5 nmol
- Shipped in 4–6 business days
PrimeTime LNA qPCR Probes
- Available with FAM, Cy® 3, Cy 5, TEX, TYE™, YAK, and HEX dyes
- High synthesis scales (250 nmol and 1 µmol) for large-scale and high throughput requirements
- Shipped in 4–6 business days