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RNA oligos

Specificity you need for your functional genomics research

All single-stranded and duplexed RNA sequences are produced using proprietary technology that delivers industry-leading RNA quality. Our specialized synthesis platforms allow us to produce the highest fidelity guides for CRISPR, duplexes for RNAi, template switching oligos for NGS, microRNAs, aptamers, and custom RNA oligos for other applications.

  • Tailor RNA oligos to your application by selecting from chimeric DNA bases, modified RNA bases, and a wide variety of modifications
  • Conduct your experiment sooner with fast turnaround times for desalted and HPLC-purified RNA oligos
  • Have complete confidence in oligos that are quality controlled by ESI-mass spectrometry
  • For your convenience every RNA oligo is deprotected and shipped ready to use

Ordering

Single-stranded RNA

Shipped dry.

ProductLengthRNA bases2' O-Methyl RNA basesChimeric DNA basesRNase-Free HPLC
100 nmole RNA Oligo5 - 60 bases$7.50 USD / base$9.50 USD / base$0.60 USD / base$75.00 USD
250 nmole RNA Oligo5 - 60 bases$9.50 USD / base$14.50 USD / base$1.00 USD / base$105.00 USD
1 umole RNA Oligo5 - 60 bases$20.00 USD / base$22.00 USD / base$2.10 USD / base$160.00 USD
5 umole RNA Oligo5 - 60 bases$60.00 USD / base$85.00 USD / base$11.50 USD / base$340.00 USD
10 umole RNA Oligo5 - 60 bases$115.00 USD / base$160.00 USD / base$20.00 USD / base$590.00 USD

Duplexed RNA

2 oligos, annealed and delivered in a single tube. Shipped dry.

ProductLengthRNA bases2' O-Methyl RNA basesChimeric DNA basesRNase-Free HPLC
100 nmole RNA Duplex Oligo5 - 60 bases$7.50 USD / base$9.50 USD / base$0.60 USD / base$75.00 USD
250 nmole RNA Duplex Oligo5 - 60 bases$9.50 USD / base$14.50 USD / base$1.00 USD / base$105.00 USD
1 umole RNA Duplex Oligo5 - 60 bases$20.00 USD / base$22.00 USD / base$2.10 USD / base$160.00 USD

Annealing fees will be applied to duplexed oligos (per duplex), as follows:

100 nmol:
$30.00 USD
250 nmol:
$40.00 USD
1 µmol:
$45.00 USD

Single-stranded RNA

Shipped dry or resuspended to your specifications. A minimum of 24 or 96 oligos required for 96- or 384-well plates, respectively.

ProductLengthRNA bases2' O-Methyl RNA basesChimeric DNA basesRNase-Free HPLC
100 nmole RNA Plate Oligo5 - 60 bases$7.50 USD / base$9.50 USD / base$0.29 USD / base$75.00 USD
250 nmole RNA Plate Oligo5 - 60 bases$9.50 USD / base$14.50 USD / base$0.49 USD / base$105.00 USD
1 umole RNA Plate Oligo5 - 60 bases$20.00 USD / base$22.00 USD / base$1.00 USD / base$160.00 USD

RNA oligos are short, single- or double-stranded synthetic RNA sequences that can be used in nearly any RNA-specific molecular biology application. Using the Oligo Entry ordering tool, you can design your sequences to contain unmodified RNA bases, 2′-O-methyl RNA bases, or chimeric DNA bases. You can select from hundreds of modifications to suit your specific research needs. (2′-O-methyl RNA is a useful RNA analog that is resistant to degradation by single-stranded ribonucleases.)

Every RNA Oligo you receive is deprotected and desalted to remove small molecule impurities. In addition, your oligos will be sequence-verified via proprietary electrospray ionization–mass spectrometry methods, and quantified twice by UV spectrophotometry to provide accurate yield measurements.

Using the RNA Oligo Entry tool

  • To enter individual RNA bases, insert "r" before the base (rA, rU, rC, rG)
  • To enter individual 2′-O-Methyl RNA bases, insert "m" before the base (mA, mU, rC, rG)
  • To quickly convert an entire oligo to RNA, enter your sequence as DNA using A, T, C, G and then click “Convert to “RNA”. All bases in your sequence will be converted to RNA (rA, rU, rC, rG)

IDT’s long-standing reputation as a leader and pioneer in custom oligo manufacturing is largely due to our vertically integrated proprietary synthesis platforms. Nearly every component of our manufacturing process is designed and developed in-house, including specialized synthesizers that can accommodate the most demanding oligo requests, and high-throughput automation systems that ensure the fastest turnaround times possible. We do not rely on third-party manufacturers for any of the machines or chemical reagents used for synthesis, allowing us to easily monitor and refine instruments and reagents with ease. Our ability to control these variables allows us to produce oligos that are unmatched in quality and consistency.

The inherent structure of RNA makes it more difficult to manufacture than DNA. However, our expertise in nucleic acid synthesis allows us to consistently achieve the highest coupling efficiencies in the industry, meaning you will always receive RNA oligos with the yield and purity that your project requires.

OligoAnalyzer® tool

Identify oligonucleotide properties, including melting temperature, hairpins, dimers and mismatches

UNAFold tool

Perform secondary structure analysis

Dilution Calculator

Find the volume needed to dilute your oligo stock to a lower concentration

Resuspension Calculator

Find the volume needed to resuspend a dry oligo to a desired concentration

The following services represent our most common offerings. If your project requires a service not listed here, contact custcare@idtdna.com, and our experts will work with you to accommodate your request.

Purification

For more demanding RNA applications, additional purification by RNase-Free HPLC purification will significantly improve oligo performance. All purified oligos up to 60 bases receive a purity guarantee following quality control by capillary electrophoresis. Learn more »

ProductRNase-Free HPLC
100 nmole RNA Oligo$75.00 USD
250 nmole RNA Oligo$105.00 USD
1 umole RNA Oligo$160.00 USD
5 umole RNA Oligo$340.00 USD
10 umole RNA Oligo$590.00 USD

Formulation

Diverse application requirements and individual researcher preferences necessitate oligos that can be delivered in unique formats. You can have your oligos prepared to your specific requirements. Learn more »

For information on available services, email custcare@idtdna.com.

Custom quality control

In addition to the free electrospray ionization–mass spectrometry analysis that is provided for your oligos, your project may require one of the more advanced analytical services listed below.

ProductAnalytical IE-HPLC pH 12.0Analytical RP-HPLC
100 nmole RNA Oligo$55.00 USD$55.00 USD
250 nmole RNA Oligo$55.00 USD$55.00 USD
1 umole RNA Oligo$55.00 USD$55.00 USD
5 umole RNA Oligo$55.00 USD$55.00 USD
10 umole RNA OligoN/A$55.00 USD
Description96 well Plate384 well Plate
Entire Plate Capillary Electrophoresis$400.00 USD$1,600.00 USD

Large-scale synthesis

Custom RNA oligos can be ordered in bulk with the same high purity, competitive pricing, and fast turnaround times you expect from IDT. To learn more about our large-scale synthesis capabilities, click here.

GMP and OEM

If you require oligos that are approved for use in molecular diagnostic applications, or if you are interested in our third-party manufacturing services, please click here.