The xGen Pre-Hybridization Capture Normalase Module uses a novel enzymatic normalization technology for generating equimolar library pools and balanced sample representation ahead of hybridization capture. This method eliminates the need for individual
library quantification and pooling of variable volumes. Instead, equal volumes are pooled during the Normalase chemistry. The resulting pool has a sample read depth with a coefficient of variation of (CV) ≤ 10%, which is more uniform than library
pools generated using qPCR quantification and fluorometric assays.
The xGen Pre-Hybridization Capture Normalase Module (Figure 1 ) can be easily integrated into standard DNA and RNA library preparation and hybridization capture protocols to improve turnaround time and loading accuracy for NGS research. The workflow does
not require a second PCR; instead, indexing and Normalase library conditioning can occur in the same reaction.
Using the xGen Pre-Hybridization Capture Normalase Module, researchers can select a broad range of library inputs into hybridization capture (100–500 ng), across multiple insert sizes (150–350 bp), while supporting multiplexing of 4–24 libraries per pool.