Targeted CRISPR/Cas9 Genome Editing

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The clustered regularly interspaced short palindromic repeat (CRISPR) system is an RNA guided genome editing tool that is revolutionizing genome engineering.

Unlike the older TALEN and zinc-finger nuclease methods, CRISPR does not require labs to be experts at protein engineering to target specific sites in the genome. With the CRISPR system, you can generate or purchase one Cas9 endonuclease for your specific organism or cell type, and target many sites by changing the short, 20 bp recognition sequence of the single-guide RNA. The result is a system that relatively easy to implement, and highly flexible compared to older methods.

CRISPR Webinars


Webinar Slides and Questions


CRISPR DECODED Articles

CRISPR-Cas9 mediated HDR: Tips for successful experimental design

Protocols for CRISPR genome editing in your model system

A novel, high-fidelity Cas9 improves CRISPR editing accuracy without sacrificing performance

A recombinant Cas9 enzyme that drastically reduces CRISPR off-target effects

CRISPR-Cpf1, an alternative to Cas9 for targeting AT-rich genomes

One-step strategy to create transgenic and KO mouse models (Easi-CRISPR) uses Megamer ssDNA donors and CRISPR RNPs

Using CRISPR genome editing for gene knockout and homology-directed repair (HDR)

3 valuable functions of a fluorescently labeled CRISPR-Cas9 tracrRNA

A simple method to detect on-target editing or measure genome editing efficiency in CRISPR experiments

The key to successful electroporation in CRISPR genome editing experiments

CRISPR-Cpf1 expands genome editing to new target sites

Simple model for point mutation correction uses ssDNA repair oligo and CRISPR-Cas9 RNP

Genome editing in C. elegans using the Alt-R® CRISPR System

Genome editing tip: A CRISPR RNA annealing step can increase editing efficiency

CRISPR genome editing: 5 considerations for target site selection

Genome editing: How stable is my CRISPR RNA:Cas9 RNP complex?

Successful CRISPR genome editing in hard-to-transfect cells (i.e., Jurkat cells)

Webinar: Alt-R® CRISPR-Cas9 System ribonucleoprotein delivery optimization

Improve your genome editing with the Alt-R® S.p. Cas9 Nuclease 3NLS and modified crRNAs

No Cas9 PAM NGG sequence in your target region for genome editing?