PEGS Boston—Protein Engineering & Cell Therapy Summit

We can’t wait to see you again this year to celebrate the 20th anniversary of PEGS-Boston! Stop by booth #500 to speak with our protein experts.

This year we will be sponsoring with Danaher corporation alongside our fellow operating companies Abcam, Beckman Coulter Life Science, Phenomenex, and SCIEX. Be sure to stop by to learn more about our combined protein engineering and antibody discovery solutions and how they can help accelerate your protein research.

Solutions for protein engineering:

• eBlocks™ Gene Fragments
• gBlocks™ & gBlocks HiFi Gene Fragments
• Gene Synthesis
• CRISPR/Cas9 Gene Editing
• CRISPR cGMP Manufacturing

Poster presentations

Engineering an improved inhibitor of 53BP1 to enhance HDR efficiency

Steve E. Glenn, Adam Brainard, Vaughn Thada, Michael A. Collingwood, Bernice Thommandru, Karthik Murugan, Christopher A. Vakulskas
Integrated DNA Technologies, Coralville, IA, USA

Precise genome editing can be achieved using a targeted nuclease that introduces a dsDNA break which can then be repaired by endogenous repair pathways. A modified variant of ubiquitin was shown to bias DNA repair machinery toward a pathway that allows for incorporation of an exogenous DNA template. Here we mutagenized and isolated ubiquitin variants that allow for highly efficient gene replacement following CRISPR cleavage in livings cells.