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Frequently asked questions (FAQs)
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I transfected the cells with Alt-R™ S.p. Cas9-GFP. When should I sort the cells by fluorescence activated cell sorting (FACS) to enrich for transfected cells?
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Can Alt-R™ S.p. Cas9-GFP be used with the same protocols as Alt-R S.p. Cas9 Nuclease V3?
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What is the difference between A.s. and L.b. Cas12a? When should I use one or the other?
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When should I consider using the glycerol-free Cas9 nuclease instead of the nucleases that include glycerol?
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When designing donor DNA for use in HDR, what are the optimal lengths of the left and right homology arms?
