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Custom Probe-Based qPCR Assays

Step 1: Order Custom Probe-Based qPCR Assay(s)
Step 2: Order Probe-Based qPCR Master Mix


ASSAY INFORMATION  #1 
 
Assay NameNotes
 
SCALE


The Standard and XL qPCR Assays have options for primer to probe ratio and Dye-Quencher combinations. The actual amount of primer (in nanomoles) that will arrive in the tube is indicated below the sequence box for each primer.
 
PRIMER 1 PRIMER 2 PRIMER TO PROBE RATIO
# Bases: # Bases: Select the ratio of primer delivery amount to the probe delivery amount by moving the sliding scale.
5' 3'
Primer 1 delivery amount:
5 nanomoles
 
5' 3'
Primer 2 delivery amount:
5 nanomoles
 
Select a Ratio
 
PROBE   5' - 3' Dye-Quencher Mod View Table
# Bases: 




5' 3'
 Probe delivery amount:2.5 nanomoles
 
 
 

Multiple Entry
How many additional Assays would you like to create?
Or
Upload your sequences
 

 
Instructions
 
  1. Provide an Assay Name. This is the name that will appear on the product label. [Required]
  2. Enter any notes or comments about the assay. [Optional]
  3. Select a scale. [Required]
  4. Please use the following instructions to enter your sequences: [Required]
    • DNA bases: type the base in uppercase (e.g. A, C, T, G).
  5. Select the desired Dye-Quencher if applicable.
  6. Enter Mixed Bases. [Optional]
    • To enter a Standard Mixed Base, simply type in the respective IUB symbol. To obtain a list of these symbols, click the Mixed Bases tab above.
  7. Enter any notes or comments about the assay. [Optional]
  8. When you are finished entering the information, click the Add to Order button. You will then be taken to the Order Review Page.


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