RNA oligos

RNA oligos are short, single- or double-stranded synthetic RNA sequences that can be used in nearly any RNA-specific molecular biology application. Using the Oligo Entry ordering tool, you can design your sequences to contain unmodified RNA bases, 2′-O-methyl RNA bases, or chimeric DNA bases. You can select from hundreds of modifications to suit your specific research needs. (2′-O-methyl RNA is a useful RNA analog that is resistant to degradation by single-stranded ribonucleases.)

Every RNA Oligo you receive is deprotected and desalted to remove small molecule impurities. In addition, your oligos will be sequence-verified via proprietary electrospray ionization–mass spectrometry methods, and quantified twice by UV spectrophotometry to provide accurate yield measurements.

Using the RNA Oligo Entry tool

• To enter individual RNA bases, insert "r" before the base (rA, rU, rC, rG)
• To enter individual 2′-O-Methyl RNA bases, insert "m" before the base (mA, mU, rC, rG)
• To quickly convert an entire oligo to RNA, enter your sequence as DNA using A, T, C, G and then click “Convert to “RNA”. All bases in your sequence will be converted to RNA (rA, rU, rC, rG)

IDT’s long-standing reputation as a leader and pioneer in custom oligo manufacturing is largely due to our vertically integrated proprietary synthesis platforms. Nearly every component of our manufacturing process is designed and developed in-house, including specialized synthesizers that can accommodate the most demanding oligo requests, and high-throughput automation systems that ensure the fastest turnaround times possible. We do not rely on third-party manufacturers for any of the machines or chemical reagents used for synthesis, allowing us to easily monitor and refine instruments and reagents with ease. Our ability to control these variables allows us to produce oligos that are unmatched in quality and consistency.

The inherent structure of RNA makes it more difficult to manufacture than DNA. However, our expertise in nucleic acid synthesis allows us to consistently achieve the highest coupling efficiencies in the industry, meaning you will always receive RNA oligos with the yield and purity that your project requires.

OligoAnalyzer^® tool

Identify oligonucleotide properties, including melting temperature, hairpins, dimers and mismatches

UNAFold tool

Perform secondary structure analysis

Dilution Calculator

Find the volume needed to dilute your oligo stock to a lower concentration

Resuspension Calculator

Find the volume needed to resuspend a dry oligo to a desired concentration

The following services represent our most common offerings. If your project requires a service not listed here, contact custcare@idtdna.com, and our experts will work with you to accommodate your request.

Formulation

Diverse application requirements and individual researcher preferences necessitate oligos that can be delivered in unique formats. You can have your oligos prepared to your specific requirements. For information on available services, email custcare@idtdna.com.

Large-scale synthesis

Custom RNA oligos can be ordered in bulk with the same high purity, competitive pricing, and fast turnaround times you expect from IDT. To learn more about our large-scale synthesis capabilities, click here.

GMP and OEM

If you require oligos that are approved for use in molecular diagnostic applications, or if you are interested in our third-party manufacturing services, please click here.

User guides and protocols