Custom rhAmpSeq Panels are highly multiplexed primer sets for targeted sequencing. Panels have been designed for and successfully tested in a wide range of animal and plant species, including human, macaque, mouse, tomato, corn, and grape.
Custom rhAmpSeq Panels are well suited for diverse targeted NGS applications, including:
- Marker-assisted selection and genomic selection in agricultural genetics and breeding
- Confirmation of on-target and off-target CRISPR gene editing experiments
- Hotspot panels for oncology, rare and inherited diseases, and other disease research
Design custom panels tailored to your research
Our expert bioinformatics team will help you design a custom, targeted sequencing panel optimized for your targets in your plant or animal species of interest.
We provide a detailed summary report for your custom panel design so you can review the results and, if necessary, iterate your design with the Design Service before ordering your Custom rhAmpSeq Panel.
Custom rhAmpSeq Panels are designed for hotspot target SNPs and indels. We do not currently offer gene scanning or tiling panels, but we expect to offer panel design services for those applications in future product releases.
Custom rhAmpSeq Design Service
The 2 key steps in our custom design bioinformatics pipeline provide performance and workflow advantages over other amplicon sequencing systems.
- Assay design involves extensive target site review and rhAmp primer design for each target, followed by comprehensive QC of each rhAmp primer to mitigate off-target effects, such as primer-dimer formation and non-specific genomic hybridization.
- Virtual assay pooling is a unique multiplex primer QC feature that further enhances the specificity of rhAmp PCR technology. It allows us to more accurately predict potential primer-dimer amplification products that could lower overall reaction efficiency and decrease the percentage of correctly mapped reads.
Custom rhAmpSeq Panels are available in 3 convenient scales to fit your experimental needs: 0.4 nmol, 4 nmol, and 8 nmol. As shown in Table 1, we recommend adjusting rhAmp primer concentrations in the first amplification reaction (Targeted rhAmp PCR 1) depending on your panel size (plexity). Table 1 shows the approximate number of reactions for each scale based on panel size and scale.
Table 1. Number of rhAmp primer reactions based on scale and panel size.