xGen® Blocking Oligos
Prevent adapter cross-hybridization by using xGen Blocking Oligos in your target capture reactions. Adapter sequences specific to a given next generation sequencing platform are ligated to all library fragments, both on-target and off-target, before enrichment. These adapter sequences can hybridize with each other during enrichment, creating a "daisy-chain" effect in which off-target fragments are captured along with on-target fragments, which can impact sequencing efficiency.
xGen Blocking Oligos bind to platform adapter sequences on a designated strand (usually the inverse of the synthetic adapter) to help prevent non-specific binding. Blocking the adapter sequences significantly improves capture performance [1,2].
All xGen Blocking Oligos are compatible with any xGen Lockdown Panel or custom xGen Lockdown Probe set. Each oligo is individually synthesized and assessed by mass spectrometry for quality control. Predesigned xGen Universal Blockers receive additional functional validation to improve performance and provide consistent results.
xGen® Universal Blockers—TS Mix
xGen Universal Blockers—TS Mix is a specially formulated blend of IDT’s industry leading universal blocking oligos for Illumina adapters. Based on proprietary technology, this ready-to-use, single tube mix blocks Illumina LT (p5, p7- 6nt & 8nt) and HT (i5, i7) adapters to deliver optimal on-target sequencing performance and overall cost-effectiveness. Both 16- and 96-reaction formats are available for convenient experimental design and use with xGen Lockdown® Panels or xGen Lockdown Probe sets.
xGen Standard Blocking Oligos
Standard blocking oligos are made-to-order oligonucleotides that target specified adapter sequences.
xGen Custom Blocking Oligos
Custom blocking oligos are available in either Universal or Standard formats.
xGen Blocking Oligos for Ion Torrent Adapters
These universal blockers are specific to the P1 and barcoded A adapters.